Filtration Hints |
Filtration Speed |
“how long is a piece of string” . . . it All depends . . . |
some Equipment Limitations |
BottleTop Filter |
Vacuum System |
Hand Vacuum Pump : can develop higher vacuum at slow filtration speeds and collapse plastic filtration vacuum bottles |
Syringe Filter |
- pressurised by thumb pressure ( syringe dispenser ) |
Prefilters |
- are used to speed up flow and SF capacity coarser layer traps large particles and helps protect the main filter material |
re “Sterility” |
This is not to be regarded as advice re TGA implications - see Chromalytic DISCLAIMER |
some methods |
disinfectants, boiling water, dry heat 300deg C ( borosilicate glass ) |
Application development |
Syringe Filter MS |
1 with Luer Lok Syringe |
Step 1 consider material compatability - filtration equipment and fluid |
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if slow |
due to large particulates |
Consider preFilter (1) 1um Glass Fibre (2) multi Layer |
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viscous solution |
- try warming or if possible dilution with solvent ( subsequent evaporation or sparging of solvent |
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too high a pressure on thumb |
- consider use of a Syringe Pump / with heater option |
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1 Initially use 0.45um as the main filter |
Larger Qantities |
Aqueous |
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Hand VacPUMP and Bottletop Filter MS and Nalgene |
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Oils /aqueous |
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Glass Filtration Equipment with HandVacPUMP |
“Chromatography Filtration” |
to protect instrumentation |
dirty samples |
1 consider 13mm SFs - check for sample loss QA/QC |
Filtration Products see Also Syringe Filters - MEMBRANE SOLUTIONS |
Filtration Material - Applications PDF |
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