more Filtration Hints 2016 |
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Vac-u-Fill Bottles PDF |
Liquid Dispensers |
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Selection of Filter Media : by size |
0.45um : 0.22um |
Syringe Filter : max Pressure 72psi (PP body ) |
Membrane Filter |
Pre Filter : protects main filter from blocking extends lifetime and can improve speed |
use coarser Glass Fibre |
some have Class Fibre |
on top of main filter |
Relative speed based on |
13 : 25 : 33mm;(50mm 90mm |
at same Pore Size um |
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- Liquid Temperature |
+20degC |
~2x the spped 1/2 the viscosity |
DON”T exceed membrane or filter holder max use Temp - generally 65degC MAX BUT CHECK ! |
Measured |
Syringe Pump : Olive Oil 25C |
50ml syringe 29mmID |
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Vacuum vs Pressure |
HandPump Vacuum 350mm = -7.0psi max developed Vac Flask Filtration |
47mm |
47mm MF Calculated |
Olive Oil |
Viscosity |
Flow is proportionall to |
heating speeds up flow |
PROBLEMS ? |
OK ! heat to help dissolve sample into “solvent” matrix |
On-Cooling the sample itself can crystallise out and completely block the filter membrane and even the Glass Frit or support base of the filter holder |
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Membrane Filter and Filter Holder |
Max Temp |
Check the MAX Temperature use for each ! |
Vac-U-Fill PDF
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Oils : some tend to soften polystyrene containers - can crack or tend to “implode”/squash under Vac |
VacuCap FastCAP Bottle-Top Filtration
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for “OILS” > the BEST |
Olive Oil; Canola, Palm oil, Cottonseed |
multi-Layer SF and pre-filters help preserver main and can improve flow |
for auto-filling from sample reservoir - fast for thin low viscosity liquids |
for thin aqueous Solutions |
Hand VAC Pump
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ALL filters tend to block up depending on particulates that actually block the filter pores |
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use with Hand Vacuum Pump MityVac 12-MV8010 |
use with Motorised Vacuum Pump VPJ0332 |
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VacuCap 90 |
VC-4621 |
0.1um for critical sterilisation |
$375 |
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10-pk |
VC-4622 |
0.2umfor sterilisation |
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Sterile / Fast |
VC-4624 |
0.45um |
$350 |
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1-5Liter volumes . . . Simple Glassware Accessory use |
The VacuCap Filter is used for sterilization or clarification of a wide variety of fluids. use for cell culture and serum-containing media liquids
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PES FastCAP 0.22um 90mm |
Other Filtration Devices |
ASK ! |
IDEAL : Use as a pre-Filter for |
Vac-U-Fill preferred for aqueous based mixes, more polar solvents |
CHROMTECH : Specialists in Chromatography |
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